Small Extracellular Vesicles Derived from Adipocytes Attenuate Intervertebral Disc Degeneration in Rats by Rejuvenating Senescent Nucleus Pulposus Cells and Endplate Cells by Delivering Exogenous NAMPT

Figure 9

Inhibition of Sirt1 attenuates the therapeutic effects of Adipo-sEVs on senescent EPCs. (a) Representative micrographs of EPCs stained with SA-β-gal in different treatment groups. SA-β-gal-positive cells are shown in blue. Scale bar, 100 μm. (b) Percentage of SA-β-gal-positive cells. per group. (c) The expression of P16 was assessed by western blotting. (d) Densitometric quantification of the relative band intensity in (c). per group. (e) Representative micrographs of EPCs stained with Alizarin Red in different treatment groups. Scale bar, 100 μm. Alizarin Red to detect mineralization. (f) Ossification-associated protein expression was assessed by Western blotting. (g) Densitometric quantification of the relative band intensity in (f). per group. (h) IF staining for Runx2 (red). DAPI was used to stain the nuclei. Scale bar, 50 μm. (i) Percentage of Runx2-positive cells. per group. ( compared with the IL-1β group; ^# compared with the IL-1β+sEV group).

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