LYAR Promotes Colorectal Cancer Progression by Upregulating FSCN1 Expression and Fatty Acid Metabolism

Figure 4

LYAR binds to the FSCN1 promoter and directly activates the expression of FSCN1. (a) A schematic diagram showing seven primer pairs spanning the FSCN1 promoter that were designed for ChIP. (b) ChIP analysis of LYAR on the FSCN1 promoter in HCT15 cells. Normal rabbit IgG served as the control. Results are shown as the (). and compared to the IgG control. (c) ChIP analysis of LYAR on the FSCN1 promoter in LYAR knockdown and control HCT15 cells. Normal rabbit IgG served as the control. Results are shown as the (). compared to the IgG control. (d) Western blot assay showing LYAR protein expression in LYAR knockdown HCT15 cells. GAPDH served as the loading control. (e) Luciferase reporter analyses of wild-type FSCN1 promoter (“Wild type”) and mutant FSCN1 promoter (“Point mutant”) in LYAR knockdown HCT15 cells. Results are shown as the (). compared with the scrambled shRNA control.

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