LYAR Promotes Colorectal Cancer Progression by Upregulating FSCN1 Expression and Fatty Acid Metabolism

Figure 6

Reduction of FSCN1 expression in CRC cells inhibits the expression of some key enzymes in fatty acid metabolism. (a) Downregulated genes () were enriched in several pathways (based on the Reactome pathway database). (b) Quantitative real-time PCR analysis of FSCN1, FASN, and SCD from FSCN1 knockdown or control HCT15 cells. Results are shown as the (). compared with the control. (c) Western blot assay showing FSCN1, FASN, and SCD protein expression in FSCN1 knockdown HCT15 cells. GAPDH served as the loading control. (d) Quantitative real-time PCR analysis of FASN, SCD, and FSCN1 in FSCN1 knockdown or control HCT15 cells from xenograft. compared with the control.

(a)

(b)

(c)

(d)