TMIGD1 Inhibited Abdominal Adhesion Formation by Alleviating Oxidative Stress in the Mitochondria of Peritoneal Mesothelial Cells

Figure 3

TMIGD1 inhibits abdominal adhesion formation. (a) IHF for TMIGD1 (green fluorescence) and CK19 (red fluorescence) in each group. ; the yellow brackets show normal peritoneum or adhesion tissue; 900x magnification. (b) Quantification of relative positive TMIGD1 expression in the CK19-marked mesothelial cells in each group. ; compared to the vector-normal control (NC) group, ; abnormal distribution, Kruskal-Wallis test. (c) IHC showing the expression of TMIGD1 in each group. ; the black arrows mark the normal peritoneum or adhesion tissue; 400x magnification. (d) TMIGD1 scores in the IHC images of each group. ; compared to the vector-NC group, ; abnormal distribution, Kruskal-Wallis test. (e) Typical gross observation of abdominal adhesion conditions in each group. ; the black arrows mark adhesion tissue. (f) Application of an objective grade and tenacity adhesion score by two independent researchers quantifies the relative adhesion severity of each group. ; compared to the vector-NC group, ; abnormal distribution, Kruskal-Wallis test.

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