TMIGD1 Inhibited Abdominal Adhesion Formation by Alleviating Oxidative Stress in the Mitochondria of Peritoneal Mesothelial Cells

Figure 4

TMIGD1 protects mesothelial cells from oxidative stress injury. (a) qPCR identified TMIGD1 expression in the NC and TMIGD1-overexpressing cell lines. ; compared to the NC, , test. (b) Western blotting identified TMIGD1 expression in the NC and TMIGD1-overexpressing cell lines; . (c) MTT assay showing the viability of mesothelial cells undergoing oxidative stress injury induced by different concentrations of H₂O₂ in the NC and TMIGD1-overexpressing cell lines. ; compared to the NC, ; test. (d) Western blotting showing the apoptosis-related protein expression of mesothelial cells undergoing oxidative stress injury induced by H₂O₂. . (e) Quantification of the apoptosis-related protein expression of mesothelial cells undergoing oxidative stress injury induced by H₂O₂. Compared to the NC, ; test. (f) The apoptosis levels of mesothelial cells examined by flow cytometry transfected with a vector of NC or TMIGD1 overexpression and treated with H₂O₂ for 24 hours (). . (g) Quantification of the apoptosis levels of mesothelial cells examined by flow cytometry. Compared to the NC treated with 500 μM of H₂O₂, ; test. (h) CK19 staining showing the severity of mesothelial cell injury in each group. ; the black arrows mark the normal peritoneum or adhesion tissue; 400x magnification. (i) The completeness rate of peritoneal mesothelial cells in each group. ; compared to the vector-NC group, ; abnormal distribution, Kruskal-Wallis test.

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