TMIGD1 Inhibited Abdominal Adhesion Formation by Alleviating Oxidative Stress in the Mitochondria of Peritoneal Mesothelial Cells

Figure 5

TMIGD1 alleviates oxidative stress in the mesothelial cells. (a) ROS levels in mesothelial cells transfected with a vector of NC or TMIGD1 overexpression and treated with H₂O₂ (). ; 200x magnification. (b) Quantification of ROS levels in mesothelial cells transfected with a vector of NC or TMIGD1 overexpression and treated with H₂O₂. Compared to mesothelial cells without the induction of H₂O₂, ; ^#compared to the NC group with the induction of 500 μM of H₂O₂, ; test. (c) IHF showing the ROS intensity in each group; the yellow brackets show normal peritoneum or adhesion tissue. ; 400x magnification. (d) Quantification of relative ROS intensity in each group. ; compared to the vector-NC group, ; abnormal distribution, Kruskal-Wallis test. (e) The MDA level in each group. ; compared to the vector-NC group, ; compared to the vector-NC group, ; abnormal distribution, Kruskal-Wallis test.

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