TMIGD1 Inhibited Abdominal Adhesion Formation by Alleviating Oxidative Stress in the Mitochondria of Peritoneal Mesothelial Cells

Figure 6

TMIGD1 alleviates oxidative stress by protecting the mitochondrial function of mesothelial cells. (a) JC-1 staining showing the mitochondrial membrane potential level of mesothelial cells. The flow cytometry scatter plot showing the distribution of cell populations with JC-1 aggregates (red) and JC-1 monomers (green); . (b) Histogram of the relative ratio of red to green fluorescence (). ; compared to the NC with the induction of H₂O₂, ; test. (c) Transmission electron microscopy scan of mitochondrial conditions in each group. ; the red arrow marks mitochondria, 10000x magnification. (d) The mitochondrial length in each group. ; compared to the vector-NC, ; abnormal distribution, Kruskal-Wallis test. (e) IHF staining of mitochondria in each group. ; the red marked HSP60, the green marked the CK19, and the blue marked the DAPI; the yellow brackets show normal peritoneum or adhesion tissue; 10000x magnification. (f) Quantification of relative HSP60 expression in each group of (e). ; compared to the vector-NC, ; abnormal distribution, Kruskal-Wallis test.

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