Research Article

Effect of Lactobacillus fermentum HFY06 Combined with Arabinoxylan on Reducing Lipid Accumulation in Mice Fed with High-Fat Diet

Table 2

Primer sequences used in the quantitative real-time PCR for cecal content gene expression analysis [19].

Organism namesPrimer sequence

Total bacteriaForward:5-ACTCCTACGGGAGGCAGCAGT-3
Reverse: 5-ATTACCGCGGCTGCTGGC-3

FirmicutesForward: 5-GCGTGAGTGAAGAAGT-3
Reverse: 5-CTACGCTCCCTTTACAC-3

BacteroidetesForward: 5-ACGCTAGCTACAGGCTTAACA-3
Reverse: 5-ACGCTACTTGGCTGGTTCA-3

Lactobacillus sp.Forward:5-CACCGCTACACATGGAG-3
Reverse: 5-AGCAGTAGGGAATCTTCCA-3

Bifidobacterium sp.Forward:5-TCGCGTCYGGTGTGAAAG-3
Reverse: 5-CCACATCCAGCRTCCAC-3