Inhibition of the NADPH Oxidase Pathway Reduces Ferroptosis during Septic Renal Injury in Diabetic Mice

Figure 6

Fer-1 effectively inhibited ferroptosis accumulation during septic renal injury in diabetic mice. The expression levels of ferroptosis-related proteins, including ASCL4, FTH1, and GPX4, were determined by western blotting (a). GPX4 levels were also detected by immunohistochemical staining (b). The GR level is indicative of the renal scavenging effect on ROS (c). Morphological features of mitochondria were measured using electron microscopy (d). Each bar represents the (). and , one-way ANOVA with Tukey’s test. Hcon: mice fed a HDF diet for 12 weeks received the same volume of solvent without LPS; HLPS: mice fed a HDF diet for 12 weeks and subjected to LPS (10 mg/kg, intraperitoneally) stimulation; Fer-1+HLPS: ferrostatin-1 (Fer-1) (10 mg/kg) was administered intraperitoneally 1 h before LPS administration.

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