PCAF Accelerates Vascular Senescence via the Hippo Signaling Pathway

Figure 2

PCAF were involved in the regulation of endothelial senescence. (a) and (b) Western blotting analysis and qualification of p53, p16, pH2A.X, and p21 protein expression and relative mRNA expression of IL-6 and IL-1β in PA-treated HUVECs transfected with scramble or PCAF siRNA. (c) and (d) Western blotting analysis and qualification of p53, p16, pH2A.X, and p21 protein expression and relative mRNA expression of IL-6 and IL-1β in PA-treated HUVECs transfected with vector or PCAF plasmid. (e) and (f) SaβG staining and quantitative analysis in HUVECs transfected with scramble or PCAF siRNA in the presence of PA or not. Scale bars, 20 μm. (g) and (h) SaβG staining and quantitative analysis in HUVECs transfected with vector or PCAF plasmid in the presence of PA or not. Scale bars, 20 μm. Data are expressed as ( for each experiment. (, , , n.s. ).

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