PCAF Accelerates Vascular Senescence via the Hippo Signaling Pathway

Figure 6

Knockdown of PCAF ameliorated vascular senescence. (a) The concentration of IL-1β in the plasma from ctrl (), aged+AAV9-Luc (), and aged+AAV9-PCAF () mice were determined by ELISA kit according to its instruction. (b) and (c) Representative SaβG staining of aortic sinus sections (b) and its qualification analysis and thoracoabdominal aorta (i). Scale bars, 20 μm. (d) and (e): The expression of senescence-associated markers (d) and inflammatory factors (e) in the aorta from each group were assessed by immunoblotting. (f) The expression of YAP and p-YAP were investigated by immunoblotting. (g) and (h) The expression of p16, p21 (g) and p-YAP (h) were detected by IHC. Scale bars, 20 μm. Data are expressed as (, , , n.s. ).

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