∆nFGF1 Protects β-Cells against High Glucose-Induced Apoptosis via the AMPK/SIRT1/PGC-1α Axis

Figure 2

∆nFGF1 relieves β-cell apoptosis in db/db mice. (a) H&E staining of pancreas tissue from mice sacrificed after intraperitoneal (i.p.) injection of ∆nFGF1 or saline for 8 weeks (left panel). The black dotted lines indicate the borders of pancreatic islets. Pancreatic islet areas were quantified using ImageJ (right panel). (b) Coimmunofluorescence staining of insulin (green) and C-caspase 3 (red) in pancreatic islets. DAPI was used to stain nuclei. The white arrows indicate insulin/C-caspase 3 double-positive β-cells. (c) Representative PCNA-positive cells in pancreas sections (left panel). The black dotted lines indicate the borders of pancreatic islets. PCNA-positive cells were quantified using ImageJ (right panel). All data are presented as . mice per group. ; n.s.: no significance.

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