∆nFGF1 Protects β-Cells against High Glucose-Induced Apoptosis via the AMPK/SIRT1/PGC-1α Axis

Figure 3

∆nFGF1 inhibits apoptosis of MIN6 cells. MIN6 cells were exposed to NG (11.1 mM) and HG+PA (33 mM HG+0.5 mM PA) in the presence or absence of ∆nFGF1 for 24 h. MIN6 cells were exposed for 24 h to HG or PA. (a) The expression of Bcl-2 was analyzed by Western blot (left panel) and quantified using ImageJ (right panel). (b) The expression of Bax and cleaved- (C-) caspase 3 in MIN6 cells under different concentrations of ∆nFGF1 was analyzed by Western blot (upper panel) and quantitated using ImageJ (lower panels). (c) The survival rate of MIN6 cells for above treatments. (d) The proliferation of MIN6 cells after treatment with FGF1^WT and ∆nFGF1. Representative immunofluorescence images of MIN6 cells stained with (e) TUNEL (green) and (f) C-caspase 3 (red). DAPI was used to stain nuclei. (g) The numbers of TUNEL-positive cells (upper panel) and the fluorescence intensity of C-caspase 3 were quantified using ImageJ (lower panel). (h) Bax, Bcl-2, and C-caspase 3 expression was analyzed by Western blot (left panel) and quantitated by ImageJ (right panels). β-Actin was used as a control. All data are presented as . NG: normal glucose; HG: high glucose; PA: palmitic acid. , , and . n.s.: no significance.

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