∆nFGF1 Protects β-Cells against High Glucose-Induced Apoptosis via the AMPK/SIRT1/PGC-1α Axis

Figure 4

∆nFGF1 induces activation of AMPK/Sitr1/PGC-1α signaling. MIN6 cells were exposed to NG (11.1 mM) and HG+PA (33 mM HG+0.5 mM PA) in the absence or presence of ∆nFGF1 for 24 h. (a) The expression of phosphorylated- (p-) AMPK and AMPK analyzed by Western blot (left panel) and quantified by ImageJ (right panel) (). (b) The expression levels of SIRT1 and PGC-1α analyzed by Western blot (left panel) and quantified by ImageJ (right panel) (). β-Actin was used as a loading control. (c, d) Immunostaining for SIRT1 (red) and PGC-1α (green) in paraffin-embedded islets. DAPI was used to stain cell nuclei. mice per group. (e) Quantitative analysis of fluorescence intensity of SIRT1 (upper panel) and PGC-1α (lower panel). All data are presented as . , , and .

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