miR-342-3p Inhibits Acute Myeloid Leukemia Progression by Targeting SOX12

Figure 3

SOX12 is miR-342-3p’s direct downstream target. (a) StarBase (URL: http://starbase.sysu.edu.cn/index.php) and TargetScan (URL: TargetScanHuman 7.2) were employed to predict miR-342-3p’s downstream targets and a Venn diagram was drawn. (b) Pathway enrichment analysis of miR-342-3p’s downstream target gene sets using the KEGG database. (c) The binding sequences of SOX12 mRNA 3UTR WT and MUT to miR-342-3p. (d) Impacts of miR-342-3p overexpression on WT-SOX12 and MUT-SOX12 luciferase activity by dual-luciferase reporter gene assay. (e) Impacts of miR-342-3p mimics or inhibitors on SOX12 protein expression by Western blot. (f) qRT-PCR detection of SOX12 mRNA expression in the plasma of 47 AML patients and healthy individuals. (g) Pearson correlation analysis of the correlation between SOX12 mRNA and miR-342-3p expression in AML plasma. , , and .

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