TLR4-SIRT3 Mechanism Modulates Mitochondrial and Redox Homeostasis and Promotes EPCs Recruitment and Survival

Figure 1

Identification of TLR4 expression in human EPCs. TLR4 expression on EPCs was detected by FACS. EPCs after 7 days culture were induced by various concentrations of LPS (1 or 10 μg/mL) for 12 h. Then the EPCs were harvested and incubated with mouse anti-TLR4 antibody, followed by FITC-labeled rabbit antimouse IgG. FACS showed that the expression rate of TLR4 in human EPCs can be upregulated by LPS from 32.77% to 63.93% (1 μg/mL) or 95.33% (10 μg/mL).