TLR4-SIRT3 Mechanism Modulates Mitochondrial and Redox Homeostasis and Promotes EPCs Recruitment and Survival

Figure 4

LPS induces the changes in SIRT3 expression and activity through TLR4. Decreased protein and deacetylase activity levels by high-dose LPS (10 μg/mL) can be abrogated significantly by TLR4 blocking in EPCs. (a) Representative western blotting assay and quantitation of the level of SIRT3 protein. Data are presented as () (b). The analysis of SIRT3 deacetylase activity by using mitochondrial fractions (). (c) Pretreatment with LPS (10 μg/mL) significantly elevated the NADPH oxidative activity in a TLR4-dependent way (). vs. control; vs. 1 μg/mL LPS.

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