TLR4-SIRT3 Mechanism Modulates Mitochondrial and Redox Homeostasis and Promotes EPCs Recruitment and Survival

Figure 8

SIRT3 ameliorates the pulmonary arteriolar remodeling after MCT-induced pulmonary arteriolar injury. Three-micrometer lung sections were stained by Masson’s Trichrome Stain, by which smooth muscle is indicated as pink (pink arrow), fibril tissues as green (green arrow), cellular nucleus as brown and erythrocytes as red. Representative histological photomicrographs of interacinar pulmonary arterioles (≤100 μm) from each group are shown and analyzed. (a) The lumen of the pulmonary intra-acinar arterioles of the control group was surrounded by thin vessel wall without obvious medium and adventicium. (a’) A muscularized interalveolar arteriole from the MCT group with smooth muscle cell circumference (pink arrow) and adventitial hypertrophy (blue arrow). (a”) A partially muscularized arteriole from MCT and EPCs treated group. (a”’) An arteriole from the Let-SIRT3(+) EPCs treated group possessing a minor muscular and fibrotic component in the vessel wall (scale bars 100 μm). (b) Quantitative analysis of pulmonary arteriole medial wall thickness 6 weeks after MCT or saline injection. Data are . vs. control, vs. MCT group.

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