Downregulation of Inflammatory Response via Nrf2/Trx1/TXNIP Axis in Oxidative Stress-Induced ARPE-19 Cells and Mouse Model of AMD

Figure 2

Effect of H₂O₂ on Nrf2, NLRP3, and related genes and proteins in ARPE-19 cells. (a) RT-PCR analysis of mRNA in the following four groups: control, H₂O₂, tBHQ, and tBHQ+H₂O₂. (b) Western blot of Nrf2, NLRP3, and related proteins in the following four groups: control, H₂O₂, tBHQ, and tBHQ+H₂O₂. (c) Quantification of Nrf2, NLRP3, and related protein expression. The data are shown as ; . vs. control; vs. control; vs. control; ^# vs. H₂O₂; ^## vs. H₂O₂; ^& vs. tBHQ; ^&& vs. tBHQ; ^&&& vs. tBHQ.

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