Research Article
Downregulation of Inflammatory Response via Nrf2/Trx1/TXNIP Axis in Oxidative Stress-Induced ARPE-19 Cells and Mouse Model of AMD
Figure 4
Effect of Nrf2 on the expression of TXNIP in ARPE-19 cell. (a) RT-PCR analysis of mRNA in the following four groups: control, H2O2, tBHQ, tBHQ+H2O2, and tBHQ+siTrx1+H2O2. (b) Western blot of NLRP3 and TXNIP proteins in the following five groups: control, H2O2, tBHQ, tBHQ+H2O2, and tBHQ+siTrx+H2O2. (c) Quantification of NLRP3 and TXNIP protein expression. The data are shown as ; . vs. control; vs. control; vs. control; # vs. H2O2; ## vs. H2O2; & vs. tBHQ; && vs. tBHQ; $ vs. tBHQ+H2O2; $$ vs. tBHQ+H2O2; $$$ vs. tBHQ+H2O2.
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