Downregulation of Inflammatory Response via Nrf2/Trx1/TXNIP Axis in Oxidative Stress-Induced ARPE-19 Cells and Mouse Model of AMD

Table 1

Primer sequences used for PCR.

Gene symbols	Forward 5–3	Reverse 5–3
Nrf2 (cell)	TTAAACCTGCGGACACTGTTG	GTATCCCAAGGCGTTCTTGTT
TXNIP	GGTCTTTAACGACCCTGAAAAGG	ACACGAGTAACTTCACACACCT
Trx1	GTGAAGCAGATCGAGAGCAAG	CGTGGCTGAGAAGTCAACTACTA
NLRP3	GATCTTCGCTGCGATCAACAG	CGTGCATTATCTGAACCCCAC
IL-1β	CCTCTCTCTAATCAGCCCTCTG	GAGGACCTGGGAGTAGATGAG
IL-18	TCTTCATTGACCAAGGAAATCGG	TCCGGGGTGCATTATCTCTAC
Caspase-1	TTTCCGCAAGGTTCGATTTTCA	GGCATCTGCGCTCTACCATC
GAPDH	GGAGCGAGATCCCTCCAAAAT	GGCTGTTGTCATACTTCTCATGG
18S	CATGTACGTTGCTATCCAGGC	CTCCTTAATGTCACGCACGAT
Nrf2 (mice)	CGGGGACGCAGTGATGTATG	TGTGTAGCTGAAGGTTCGGTTA
TXNIP	GGCCGGACGGGTAATAGTG	AGCGCAAGTAGTCCAAAGTCT
Trx1	TTCCCTCACCTCTAAGACCCT	GTCAGGCTCTTCCACTCATCTAT
NLRP3	TGACCCTTATGACCAGTCCTTT	GTCAGGCTCTTCCACTCATCTAT
IL-1β	GAAATGCCACCTTTTGACAGTG	TGGATGCTCTCATCAGGACAG
IL-18	CTGTGGACATATTCTGCAAGGG	GCATGTACCACTGGACATCAGAT
Caspase-1	ATTACCCGCCCGAGAAAGG	CATGAGTGTGGCTAGATCCAAG
GAPDH	AGGTCGGTGTGAACGGATTTG	GGGGTCGTTGATGGCAAC
18S	GCTGGAGGACTCATGTTCAAC	GCACCACATCGGTAGGTCTTAAA