Docosahexaenoic Acid-Enhanced Autophagic Flux Improves Cardiac Dysfunction after Myocardial Infarction by Targeting the AMPK/mTOR Signaling Pathway

Figure 4

DHA-enhanced autophagic flux in hypoxia-treated NMCs. (a) LC3-II, p62, and GAPDH protein levels in NMCs by western blotting. (b and c) Quantification of LC3-II/GAPDH and P62/GAPDH. Data are . and vs. control, ^# and ^## vs. hypoxia, ^§ and ^§§ vs. hypoxia+DHA, ^{^} vs. hypoxia+DHA+CQ, . (d) Double immunofluorescence of LC3 (red) and p62 (green) in NMCs (scale bar 10 μm). (e and f) Corresponding quantitative analysis of LC3-II and p62 fluorescence intensity. Data are . vs. control, ^# vs. hypoxia, ^§§§ vs. hypoxia+DHA, .

(a)

(b)

(c)

(d)

(e)

(f)