Docosahexaenoic Acid-Enhanced Autophagic Flux Improves Cardiac Dysfunction after Myocardial Infarction by Targeting the AMPK/mTOR Signaling Pathway

Figure 6

DHA-enhanced autophagic flux decreased myocardial apoptosis in vitro and in vivo. (a) Apoptosis of NMCs by TUNEL staining (red; original magnification ×200; scale bar 50 μm). (b) Quantitative analysis of panel A. Data are . vs. control, ^## vs. hypoxia, ^§§ vs. hypoxia+DHA, ^{^} vs. hypoxia+DHA+CQ, . (c and d) Western blots of LC3-II, p62, and GAPDH in hypoxia-treated NMCs. Data are . 1 vs. control, ^## vs. hypoxia, ^§§ vs. hypoxia+DHA, . (e and f) Western blots of LC3-II, p62, and GAPDH in post-MI mice. Data are . vs. sham, ^## vs. MI, ^§§§ vs. MI+DHA, ^{^} vs. MI+DHA+CQ, .

(a)

(b)

(c)

(d)

(e)

(f)