The Effect of the NF<i>κ</i>B-USP9X-Cx43 Axis on the Dynamic Balance of Bone Formation/Degradation during Ossification of the Posterior Longitudinal Ligament of the Cervical Spine

<div>USP9X regulated Cx43-associated ossification of primary ligament fibroblasts. (a) Overexpression of USP9X in ligament fibroblasts resulted in Cx43 upregulation, p65 reduction in the cytosol, and p65 accumulation in nuclei, which were reversed by Cx43 KD in ligament fibroblasts overexpressing USP9X. (b) Overexpression of USP9X increased ALP activity which was significantly reduced by Cx43 KD. (c) USP9X KD in ligament fibroblasts resulted in a Cx43 decrease, p65 accumulation in the cytosol, and p65 reduction in nuclei, which were reversed by Cx43 overexpression. (d) USP9X KD decreased ALP activity which was significantly enhanced by Cx43 overexpression. 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Oxidative Medicine and Cellular Longevity

fig3

Figure 3

Figure 3: The Effect of the NF<i>κ</i>B-USP9X-Cx43 Axis on the Dynamic Balance of Bone Formation/Degradation during Ossification of the Posterior Longitudinal Ligament of the Cervical Spine 

Figure 3 | The Effect of the NFκB-USP9X-Cx43 Axis on the Dynamic Balance of Bone Formation/Degradation during Ossification of the Posterior Longitudinal Ligament of the Cervical Spine 