The Effect of the NF<i>κ</i>B-USP9X-Cx43 Axis on the Dynamic Balance of Bone Formation/Degradation during Ossification of the Posterior Longitudinal Ligament of the Cervical Spine

<div>NF-<i>κ</i>B p65 mediated the transcription of USP9X in primary ligament fibroblasts. (a) Ligament fibroblasts were treated with either TNF-<i>α</i> (10 ng/ml) or PDTC (10 <i>μ</i>M) for 24 h. Western blots showed that TNF-<i>α</i> treatment caused a significant USP9X increase, p65 reduction in the cytosol, and p65 accumulation in nuclei. PDTC treatment caused a significant USP9X decrease, p65 accumulation in the cytosol, and p65 reduction in nuclei. (b) qPCR results showed that TNF-<i>α</i> treatment caused a significant USP9X increase at the mRNA level, while PDTC treatment resulted in significant downregulation of USP9X. (c) USP9X promoter transcription activity was enhanced by TNF-<i>α</i> treatment but decreased by PDTC treatment. 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Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: The Effect of the NF<i>κ</i>B-USP9X-Cx43 Axis on the Dynamic Balance of Bone Formation/Degradation during Ossification of the Posterior Longitudinal Ligament of the Cervical Spine 

Figure 4 | The Effect of the NFκB-USP9X-Cx43 Axis on the Dynamic Balance of Bone Formation/Degradation during Ossification of the Posterior Longitudinal Ligament of the Cervical Spine 