Arterial Pulsatility Augments Microcirculatory Perfusion and Maintains the Endothelial Integrity during Extracorporeal Membrane Oxygenation via hsa_circ_0007367 Upregulation in a Canine Model with Cardiac Arrest

Figure 5

hsa_circ_0007367 (UBAP2) is needed for the pulsatility-mediated ZO-1 and occludin upregulation. (a) The distribution of ZO-1 in PMVECs treated with control siRNA or siRNA targeting circUBAP2, followed by 6-hour exposure to pulsatile flow; (b) the distribution of occludin in PMVECs treated with control siRNA or siRNA targeting circUBAP2, followed by 6-hour exposure to pulsatile flow; (c) the expressions of ZO-1 and occludin detected with western blotting in PMVECs treated with control siRNA or si-circUBAP2, followed by 6-hour exposure to pulsatile flow or nonpulsatile flow; (d) the expression of ZO-1 mRNA in PMVECs treated with control siRNA or si-circUBAP2, followed by 6-hour exposure to different flow patterns; (e) the expression of occludin mRNA in PMVECs treated with control siRNA or si-circUBAP2, followed by 6-hour exposure to different flow patterns. ; . Abbreviations: ZO-1: zonula occludens-1; DAPI: 4,6-diamidino-2-phenylindole; si: small interfering RNA; Ctrl: control; PMVEC: pulmonary microvascular endothelial cell.

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