Arterial Pulsatility Augments Microcirculatory Perfusion and Maintains the Endothelial Integrity during Extracorporeal Membrane Oxygenation via hsa_circ_0007367 Upregulation in a Canine Model with Cardiac Arrest

Figure 6

hsa_circ_0007367 (UBAP2) is required for the suppression of endothelial adhesion and inflammation mediated by pulsatile flow. (a) The expressions of endothelial adhesive molecules, NF-κB, and eNOS proteins using western blotting in PMVECs treated with control siRNA or si-circUBAP2, followed by 6-hour exposure to pulsatile flow or nonpulsatile flow; (b) the expression of NF-κB mRNA in PMVECs treated with different siRNAs and different flow patterns; (c) the expression of eNOS mRNA in PMVECs treated with different siRNAs and different flow patterns. ; . Abbreviations: ZO-1: zonula occludens-1; DAPI: 4,6-diamidino-2-phenylindole; si: small interfering RNA; Ctrl: control; eNOS: endothelial nitric oxide synthases; PMVEC: pulmonary microvascular endothelial cell.

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