Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation

Figure 1

CAL attenuated DOX-induced H9c2 cell injury. (a) Chemical structure of CAL. (b) Cytotoxicity of DOX towards H9c2 cells. Cells were treated with 0-20 μM DOX for 24 h, and cell viability was detected by CCK-8 assay. (c) DOX increased LDH level. (d) Cells were treated with 0-160 μM CAL for 24 h, and cell viability was detected by CCK-8 assay. (e) LDH level was measured in CAL-treated H9c2 cells. (f) CAL improved cell viability in DOX-treated H9c2 cells. (g) CAL decreased LDH release in DOX-treated H9c2 cells. (h) Morphological observation of H9c2 cells. All data were represented as , . , , and compared with the control group, ^#, ^##, and ^### compared with the DOX group.

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