Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation

Figure 2

Effects of CAL on DOX-induced mitochondrial oxidative stress injury in H9c2 cells. (a) ROS generation in H9c2 cells was assayed by 2,7-dichlorofluorescein–diacetate (DCFH2–DA) staining. Images were obtained using a fluorescence microscope (magnification, ×100; scale bar, 100 μm) with DCF-positive cells were green. (b) Quantitative analysis of DCF fluorescence intensity (). (c) Fluorescence intensity measured by flow cytometry. (d) Overlay flow cytometry histograms of cultures. (e) Representative images of mitochondrial membrane potential in H9c2 cells (magnification, ×100; scale bar, 100 μm). (f) Mitochondrial membrane potential measured by flow cytometry. (g) Quantitative analysis of JC-1 fluorescence intensity (). All data were represented as . , , compared with the control group, ^#, ^##, ^### compared with the DOX group.

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