Research Article

Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation

Figure 3

CAL prevented DOX-induced cardiomyocyte pyroptosis in H9c2 cells. (a) Expressions of NLRP3, ASC, caspase-1, IL-1β, IL-18, and GSDMD in H9c2 cells. Protein expression was examined by western blotting. (b) Content of IL-18 in H9c2 cell supernatant (). (c) Content of IL-1β in H9c2 cell supernatant (). (d) and (e) Quantitative analysis of NLRP3 and caspase-1 expression (). (f) and (g) Representative immunofluorescent staining of NLRP3 and caspase-1. All data were represented as . vs. control group, #, ##, ### vs. DOX group.
(a)
(b)
(c)
(d)
(e)
(f)
(g)