Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation

Figure 4

Activation of NLRP3 with nigericin and overexpression of NLRP3 reversed the antipyroptotic effects of CAL in vitro. (a) and (b) Representative immunofluorescent staining of NLRP3 and caspase-1. (c) NLRP3 activation by nigericin abolished CAL anti-pyroptotic effects in vitro. Representative Western blot images of NLRP3, ASC, Caspase-1, IL-1β, IL-18, and GSDMD in H9c2 cells. (d) Forced overexpression of NLRP3 reversed CAL antipyroptotic effects in vitro. Protein expressions of NLRP3, ASC, caspase-1, IL-1β, IL-18, and GSDMD in H9c2 cells were examined by Western blotting.

(a)

(b)

(c)

(d)