Calycosin Alleviates Doxorubicin-Induced Cardiotoxicity and Pyroptosis by Inhibiting NLRP3 Inflammasome Activation

Figure 6

CAL suppressed DOX-induced cardiomyocyte pyroptosis in mice. (a) Representative TUNEL staining of mice heart. The representative TUNEL positive cells were indicated with arrowhead. (b) Statistics analysis was performed to quantify the cardiomyocyte death (). (c) Representative images of NLRP3 immunohistochemistry. The representative NLRP3 positive cells were indicated with arrowhead. (d) Quantitative immunohistochemistry analysis of NLRP3 (). (e) Representative Western blot images of NLRP3, ASC, caspase-1, IL-1β, IL-18, and GSDMD expression in mouse myocardium. (f) Quantitative analysis of NLRP3, ASC, caspase-1, IL-1β, IL-18, and GSDMD in mouse myocardium (). GAPDH was used as loading control. Data were expressed as . and compared with the control group, ^#, ^##, and ^### compared with the DOX group.

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