Mesangial Cell–Derived Exosomal miR-4455 Induces Podocyte Injury in IgA Nephropathy by Targeting ULK2

Figure 4

Effects of miR-4455 and ULK2 mRNA 3UTR binding and miR-4455 mimics combined with ULK2 overexpression plasmid transfection on podocytes activity and apoptosis. (a) Dual luciferase reporter gene assay for miR-4455 and ULK2 mRNA 3UTR binding. (b) Effect of miR-4455 mimics on the ULK2 expression in human podocytes revealed by western blotting. (c) Detection of luciferase activity of miR-4455 mimics combined with wild type or mutant ULK2 transfection. (d) Effect of miR-4455 mimics combined with ULK2 overexpression plasmid transfection on the ULK2 expression in human podocytes revealed by western blotting. (e) Effect of miR-4455 mimics combined with ULK2 overexpression plasmid transfection on human podocytes activity by CCK8. (f) Effect of miR-4455 mimics combined with ULK2 overexpression plasmid transfection on apoptosis of human podocytes by flow cytometry (, ns means ).

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