IL-37 Expression in Patients with Abdominal Aortic Aneurysm and Its Role in the Necroptosis of Vascular Smooth Muscle Cells

Figure 4

Inhibition of RIPK3 could reduce the HASMC damage. (a) The expression of RIPK3 genes in the AAA was detected at mRNA by RT-PCR (). (b, i) Immunoblotting observation of the RIPK3 expression in the vascular tissues. (c) Immunoblotting analysis. (d) The expression of RIPK3 genes in the HASMCs () was detected at mRNA by RT-PCR. (e, f) Immunoblotting observation of the RIPK3 expression in HASMCs. (g) Flow cytometry to detect the necroptosis (as indicated by a green arrow). (h) CCK8 was used to detect the cell viability. Values are presented as the . All experiments were repeated thrice, each time with . , , , and .

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