IL-37 Expression in Patients with Abdominal Aortic Aneurysm and Its Role in the Necroptosis of Vascular Smooth Muscle Cells

Figure 5

rIL-37 reduces the damage of HASMC by AngII. (a, b) Immunoblotting observation of the IL-37 expression in HASMCs. (c, d) The expression of RIPK3 IL-1β genes in the HASMCs () was detected at mRNA by RT-PCR. (e) Immunoblotting observation of RIPK3, NLRP3, and IL-1β in HASMCs. (f) Immunoblotting analysis. (g) Flow cytometry to detect necroptosis (as indicated by a green arrow). (h) CCK8 was used to detect cell viability. Values are presented as . All experiments were repeated thrice, each time with , , , , and .

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