Inhibition of the TLR/NF-κB Signaling Pathway and Improvement of Autophagy Mediates Neuroprotective Effects of Plumbagin in Parkinson’s Disease

Figure 3

Plumbagin suppressed the activation of the TLR/NF-κB signaling pathway in BV2 cells induced by MPP+ or LPS. (a) Western blotting analysis of TLR2 and TLR4 in the BV2 cells treated with vehicle, MPP+, PL+ MPP+, and PL. (b) Quantitative data of the TLR2 protein levels in the BV2 cells treated with vehicle, MPP+, PL+ MPP+, and PL. (c) Quantitative data of the TLR4 protein levels in the BV2 cells treated with vehicle, MPP+, PL+ MPP+, and PL. (d) Western blotting analysis of NF-κB p65 in the BV2 cells with the vehicle, MPP+, PL+ MPP+, and PL. (e) Quantitative data of the NF-κB p65 protein levels in the BV2 cells treated with vehicle, MPP+, PL+ MPP+, and PL. (f) Western blotting analysis of TLR2 and TLR4 in the BV2 cells treated with vehicle, LPS, PL+ LPS, and PL. (g) Quantitative data of the TLR2 protein levels in the BV2 cells treated with vehicle, LPS, PL+ LPS, and PL. (h) Quantitative data of the TLR4 protein levels in the BV2 cells treated with vehicle, LPS, PL+ LPS, and PL. (i) Western blotting analysis of NF-κB p65 in the BV2 cells treated with vehicle, LPS, PL+ LPS, and PL. (j) Quantitative data of the NF-κB p65 protein levels in the BV2 cells treated with vehicle, LPS, PL+ LPS, and PL. (k) The quantification of the mRNA levels of TNF-α, IL-1β, and IL-6 in the BV2 cells treated with vehicle, MPP+, PL+ MPP+, and PL was examined by RT-qPCR (). (l) The quantification of the mRNA levels of TNF-α, IL-1β, and IL-6 in the BV2 cells treated with vehicle, LPS, PL+ LPS, and PL was examined by RT-qPCR (). Data is expressed as the , , , and .

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