Inhibition of the TLR/NF-κB Signaling Pathway and Improvement of Autophagy Mediates Neuroprotective Effects of Plumbagin in Parkinson’s Disease

Figure 5

Plumbagin improved autophagy in the PD models of SH-SY5Y and PC12 cells induced by MPP+. (a) The effects of different doses of plumbagin on cell viability in SH-SY5Y cells. SH-SY5Y cells were pretreated with vehicle or plumbagin (0.001 μM, 0.01 μM, and 0.1 μM) for 24 h, after which MPP+ (1 mM) was added to the cell culture plate, and the mixture was incubated for another 24 h. Cell viability was assessed by the CCK-8 assays. (b) The effects of different doses of plumbagin on cell viability in PC12 cells. PC12 cells were pretreated with vehicle or plumbagin (0.001 μM, 0.01 μM, and 0.1 μM) for 24 h, after which MPP+ (500 μM) was added to the cell culture plate, and the mixture was incubated for another 24 h. Cell viability was assessed by using the CCK-8 assay. (c) Western blotting analysis of LC3, p62, and p-mTOR in the SH-SY5Y cells treated with vehicle, MPP+, PL+ MPP+, and PL. (d) Quantitative data of the LC3-II/LC3-I, p62, and p-mTOR protein levels in the SH-SY5Y cells treated with the vehicle, MPP+, PL+ MPP+, and PL. (e) Western blotting analysis of LC3, p62, and p-mTOR in the PC12 cells-induced treatment with the vehicle, MPP+, PL+ MPP+, and PL. (f) Quantitative data of the LC3-II/LC3-I, p62, and p-mTOR protein levels in the PC12 cells treated with the vehicle, MPP+, PL+ MPP+, and PL. Data are expressed as the (). , , and .

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