sPmel17 Secreted by Ultraviolet B-Exposed Melanocytes Alters the Intercellular Adhesion of Keratinocytes

Figure 2

Expression profiles of FHL2 and E-cad in KCs treated with the CM from UVB-exposed or UVB-unexposed MCs. Primary human epidermal KCs were isolated from juvenile foreskin tissues, then treated with the CM from MCs, which had been exposed or unexposed to 30 mJ/cm² UVB. (a) qRT-PCR was performed to measure mRNA levels of FHL2 and CDH1 (encoding E-cad) in KCs treated with the CM from UVB-exposed (MC-CM/UVB) or UVB-unexposed (MC-CM/Sham) MCs. The results were normalized to the housekeeping gene GAPDH. The data represent from 3 independent experiments. ; . (b) Protein levels of FHL2 and E-cad were analyzed by western blotting. Representative blots are shown on the left. The histograms show the densitometric quantification of data with from 3 independent experiments. ; . (c) Representative images of immunostaining for F-actin (labeled by FITC-phalloidin; upper panel) and E-cad (lower panel). Nuclei were counterstained with DAPI (blue). Histograms (on the right) show the length-to-width ratio and the fluorescence intensity of 20 cells. Data represent of three independent experiments. . Scale  μm.

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