sPmel17 Secreted by Ultraviolet B-Exposed Melanocytes Alters the Intercellular Adhesion of Keratinocytes

Figure 4

Silencing of Pmel17 in MCs by lentivirus-based shRNA fails to affect the expression of FHL2 and E-cad and the cytoskeletal remodeling of KCs. (a) MCs were transfected with shRNA #3 and then were irradiated with 30 mJ/cm² UVB. Western blotting was performed to confirm the protein levels of Pmel17. The histogram shows the densitometric quantification of data with from 3 independent experiments. ; . (b) KCs were treated with CM from MCs transfected with or without Pmel17 shRNA (MC-CM/shPmel17-2) or irradiated with or without 30 mJ/cm² UVB. Western blotting was performed to detect the protein levels of FHL2 and E-cad; representative blots are shown on the left. The histograms show the densitometric quantification of data with from 3 independent experiments. ; . (c) Representative images of immunostaining for F-actin (green, upper panels) and E-cad (green, lower panels). DAPI (blue) was used to visualize cell nuclei. Scale  μm. Histograms (on the right) show the length-to-width ratio and the fluorescence intensity of 20 cells. Data represent of three independent experiments. ns: no significant difference.

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