sPmel17 Secreted by Ultraviolet B-Exposed Melanocytes Alters the Intercellular Adhesion of Keratinocytes

Figure 5

Expression profiles of FHL2 and sPmel17 in UVB-exposed mouse tail skin. (a) The tail skin of Dct-LacZ transgenic mice was irradiated with 500 mJ/cm² UVB three times a week for a total of 9 times over 3 weeks. Representative dermoscope images of UVB-exposed mouse skin are shown in the upper panels. The tail skin was biopsied for cryosectioning and was stained by X-gal solution (lower panels). Histograms (on the right) show the number of LacZ-positive cells in 10 fields. Data represent of three independent experiments. . Scale  μm. (b) Representative images of immunofluorescence costaining of FHL2 (green) and sPmel17 (red) in UVB-exposed (lower panels) or UVB-unexposed (upper panels) mouse tail skin. Higher magnifications of the boxed areas in the merged images are shown on the far right. (c) Histograms show the fluorescence intensity of 10 fields. Data represent of three independent experiments. . Scale  μm.

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