HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis

Figure 10

(a) Inflammation level was significantly downregulated in HBO1 knockdown group. The cells were seeded in a 15 mm petri dish. After adherence, cells were cultured for 24 h. The cells were washed three times with PBS and fixed with paraformaldehyde for 15 min at room temperature. 50 μl of 5% goat serum was added to each well of the cell culture plate for 30 min, 50 μl/well of primary antibodies. The next day, the primary antibody was removed. After washing 3 times with PBS, the corresponding fluorescently labeled secondary antibody was added and incubated for 1 h. Finally, nuclei were stained with DAPI. Cell information was collected under an inverted microscope and confocal microscope. (b) The expression of NF-κB signaling and NLRP3 were reduced compared to LPS treatment group. Data are showed as . Asterisks indicate significant differences ().

(a)

(b)