HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis

Figure 2

(a) The expression area of α-SMA in the HBO1 knockdown group was decreased. The sections were then incubated with 10% normal goat serum and incubated for 1 h for blocking. Primary antibodies were added and incubated overnight at 4°C. Tissue samples were incubated with secondary antibodies. Then, diaminobenzidine (DAB) color development was carried out; the degree of color development was controlled under optical microscope; the samples were dehydrated with gradient ethanol, transparent in xylene, and sealed with neutral gum. Microscopic observation was then performed. (b) Collagen-1, CTGF, α-SMA, and vimentin were significantly decreased in HBO1 knockdown group (mRNA level). TRIzol solution was added into the cells or tissues; chloroform was then added at room temperature for 3-5 min. RNA pellets were collected and reverse-transcribed into cDNA. RT-PCR assays were then performed. (c) The expression of collagen-1, CTGF, and fibronectin was downregulated in the HBO1 knockdown group by immunohistochemistry. (d) TGF-β expression was significantly decreased in the HBO1 knockdown group. (e) The expression of F4/80 was decreased in HBO1 knockdown group. Data are showed as . Asterisks indicate significant differences ().

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