HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis

Figure 8

(a) The effect of HBO1 knockdown on CCL4-induced liver cytotoxicity. (b) The proportion of cells in S phase was increased. Cells were collected by centrifugation. The supernatant was then discarded. The cell pellet was homogeneously pipetted, and the cell samples were fixed with fixative solution at 4°C overnight. Cell cycle was detected by flow cytometry. (c) Hepatocyte apoptosis was significantly reduced in the HBO1 knockdown group. (d) The mitochondrial membrane potential was decreased in the HBO1 knockdown group. (e) HBO1 significantly decreased the expression of caspase-1, caspase-3, and Bax and upregulated the expression of Bcl-2. (f) HBO1 knockdown significantly attenuated CCL4-induced cytotoxicity in the AML12 cell line. (g) HBO1 knockdown treatment reduced the rate of cell apoptosis. (h) HBO1 knockdown decreased the expression of Bax, caspase-3, and caspase-1 and increased Bcl-2 expression. Data are showed as . Asterisks indicate significant differences ().

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)