HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis

Figure 9

(a) HBO1 knockdown alleviated CCL4-induced oxidative stress in hepatocytes. Oxidative stress levels were analyzed using a commercially available kit according to the kit’s instructions. (b) HBO1 knockdown alleviated CCL4-induced oxidative stress in the AML12 cells. (c) HBO1 knockdown upregulated NRF2 expression. The cells were seeded in a 15 mm petri dish. After adherence, cells were cultured for 24 h. The cells were washed three times with PBS and fixed with paraformaldehyde for 15 min at room temperature. 50 μl of 5% goat serum was added to each well of the cell culture plate for 30 min, 50 μl/well of primary antibodies. The next day, the primary antibody was removed. After washing 3 times with PBS, the corresponding fluorescently labeled secondary antibody was added and incubated for 1 h. Finally, nuclei were stained with DAPI. Cell information was collected under an inverted microscope and confocal microscope. Data are showed as . Asterisks indicate significant differences ().

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