Cardioprotective Effect of circ_SMG6 Knockdown against Myocardial Ischemia/Reperfusion Injury Correlates with miR-138-5p-Mediated EGR1/TLR4/TRIF Inactivation

Figure 1

Bioinformatic analysis of I/R injury-related genes and upregulated expression of EGR1 in myocardial tissues of I/R mice. (a) Venn diagram analysis of the significantly upregulated genes in myocardial tissues of I/R mice in the GSE4105 and GSE67308 datasets. (b) A PPI network of the 54 intersected genes. The redder color of the circle where the gene is reflects the higher core degree, and the bluer color indicates lower core degree. (c) A box plot of the expression data of EGR1 in normal () and disease samples () in the GSE4105 dataset. (d) Detection of LVSP, LVDP, +dp/dtmax, and -dp/dtmax related to cardiac function in I/R and sham-operated mice. (e) LVFS% and LVEF% detected by electrocardiogram in I/R and sham-operated mice. (f) TTC staining of myocardial tissues of I/R and sham-operated mice. (g) HE staining of myocardial tissues of I/R and sham-operated mice. (h) LDH and CK levels in arterial blood and MDA and SOD levels in myocardial tissues in I/R and sham-operated mice. (i) EGR1 mRNA expression determined by qRT-PCR in myocardial tissues of I/R and sham-operated mice. (j) EGR1 protein expression determined by Western blot analysis in myocardial tissues of I/R and sham-operated mice. vs. the sham-operated mice or control cells. for mice following each treatment.

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