Cardioprotective Effect of circ_SMG6 Knockdown against Myocardial Ischemia/Reperfusion Injury Correlates with miR-138-5p-Mediated EGR1/TLR4/TRIF Inactivation

Figure 2

Knockdown of EGR1 attenuates myocardial injury in I/R mice. (a) Silencing efficiency of sh-EGR1 confirmed by qRT-PCR in myocardial tissues of sham-operated mice or I/R mice in the presence/absence of sh-EGR1 treatment. (b) Detection of LVSP, LVDP, +dp/dtmax, and -dp/dtmax related to cardiac function in sham-operated mice or I/R mice in the presence/absence of sh-EGR1 treatment. (c) LVFS% and LVEF% detected by electrocardiogram in sham-operated mice or I/R mice in the presence/absence of sh-EGR1 treatment. (d) Activity LDH and CK in the arterial blood and SOD and MDA levels in the myocardial tissues of sham-operated mice or I/R mice in the presence/absence of sh-EGR1 treatment. (e) TTC staining of myocardial tissues of sham-operated mice or I/R mice in the presence/absence of sh-EGR1 treatment. (f) HE staining of myocardial tissues of sham-operated mice or I/R mice in the presence/absence of sh-EGR1 treatment. (g) Apoptosis of cardiomyocytes in myocardial tissues of sham-operated mice or I/R mice in the presence/absence of sh-EGR1 treatment, as assessed by TUNEL assay. (h) Protein expression of Bax, cleaved caspase-3, and Bcl-2 determined by Western blot analysis in myocardial tissues of sham-operated mice or I/R mice in the presence/absence of sh-EGR1 treatment. vs. the sham-operated mice. # vs. I/R mice treated with sh-NC. for mice following each treatment.

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