Cardioprotective Effect of circ_SMG6 Knockdown against Myocardial Ischemia/Reperfusion Injury Correlates with miR-138-5p-Mediated EGR1/TLR4/TRIF Inactivation

Figure 4

Knockdown of circ_SMG6 alleviates myocardial I/R injury by upregulating miR-138-5p and reducing EGR1 expression in vivo. I/R mice were treated with sh-circ_SMG6, sh-circ_SMG6 + antagomir-miR-138-5p, agomir-miR-138-5p, or agomir-miR-138-5p + oe-EGR1. (a) Expression of circ_SMG6, miR-138-5p, and EGR1 measured by qRT-PCR in myocardial tissues of mice in each group. (b) Detection of LVSP, LVDP, +dp/dtmax, and -dp/dtmax related to cardiac function in I/R mice. (c) LVFS% and LVEF% detected by electrocardiogram in I/R mice. (d) Activity of LDH and CK in the arterial blood of I/R mice. (e) TTC staining of myocardial tissues of I/R mice. (f) Apoptosis of cardiomyocytes in myocardial tissues of I/R mice assessed by TUNEL assay. vs. the I/R mice treated with sh-NC or agomir-NC. # vs. the I/R mice treated with sh-circ_SMG6. & vs. the I/R mice treated with agomir-miR-138-5p. for mice following each treatment.

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