Cardioprotective Effect of circ_SMG6 Knockdown against Myocardial Ischemia/Reperfusion Injury Correlates with miR-138-5p-Mediated EGR1/TLR4/TRIF Inactivation

Figure 5

circ_SMG6 silencing impedes H/R-induced cardiomyocyte injury and apoptosis by enhancing miR-138-5p-mediated EGR1 inhibition. Normal cells served as control and H/R-induced HL-1 cells were transfected with sh-circ_SMG6, sh-circ_SMG6 + miR-138-5p inhibitor, miR-138-5p mimic, or miR-138-5p mimic + oe-EGR1. (a) Expression of circ_SMG6, miR-138-5p, and EGR1 measured by qRT-PCR in myocardial tissues of mice in each group. (b) Detection of LDH and SOD activity and MDA level in the supernatant of H/R-induced HL-1 cells. (c) HL-1 cell viability measured by MTT assay. (d) Flow cytometric analysis of HL-1 cell apoptosis rate. (e) Protein expression of apoptosis-related Bax, caspase-3, cleaved caspase-3, and Bcl-2 determined by Western blot analysis in H/R-induced HL-1 cells. vs. the normal cells. # vs. the H/R-treated HL-1 cells transfected with sh-NC or NC mimic. & vs. the H/R-treated HL-1 cells transfected with sh-circ_SMG6. $ vs. the H/R-treated HL-1 cells transfected with miR-138-5p mimic. Cell experiments were conducted three times independently.

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