Never-in-Mitosis A-Related Kinase 8 (NEK8) Regulates Adipogenesis, Glucose Homeostasis, and Obesity

Figure 2

NEK8 physically associated with TAZ. (a) 3T3-L1 cells were transiently transfected with the NEK8 overexpression plasmid with 0, 0.5, 1, and 2 μg and then incubated for an additional 48 hours, and the cell lysates were collected to determine the NEK8 and TAZ level by western blot assay and β-actin as a loading control. (b, c) Co-immunoprecipitation (Co-IP) was conducted to detect the interaction between NEK8 and TAZ, and IgG was used as a control. (d) Confocal immunofluorescence staining was used to detect the colocalization of NEK8 and TAZ.

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