Nur77 Deficiency Exacerbates Macrophage NLRP3 Inflammasome-Mediated Inflammation and Accelerates Atherosclerosis

Figure 4

ox-LDL activates NLRP3 inflammasome and induces IL-1β-mediated inflammation in PMs. PMs were treated with ox-LDL at a gradient concentration of 0, 25, 50, and 100 μg/ml for 24 h and then were collected for analysis. (a) The LDH activity in supernatant was assessed by LDH Cytotoxicity Assay Kit. The expression levels of NLRP3 inflammasome-related protein NLRP3 (b), c-caspase-1 (c), c-IL-1β (d), and GSMD-N (e) were determined by Western blotting. (f) The relative mRNA level of NLRP3 and IL-1β; P <0.05 compared with the control group. Relative protein levels of NLRP3 (g), c-caspase-1 (h), c-IL-1β (i) and GSMD-N (j). n = 3. Values are shown as the mean ± SEM. P <0.05.

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